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induro rt  (New England Biolabs)


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    Structured Review

    New England Biolabs induro rt
    Induro Rt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/induro+rt/Induro+Reverse+Transcriptase/pmc13129541-148-12-14
    Average 98 stars, based on 135 article reviews
    induro rt - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    In Vitro:

    Article Title: Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT
    Article Snippet: .. An in vitro transcribed 7.6 kb HCV small genome RNA fragment was incubated in 1X reaction buffer for UltraMarathonRT ® (RNAConnect TM Cat. No. R1002), Maxima TM H Minus (ThermoScientific TM , Cat#K1652), SuperScript TM IV (Invitrogen TM , Cat#18091300), and Induro ® RT (NEB TM , CatM0681) at their recommended reaction temperatures for 0, 10, 20, and 30 min respectively. .. Samples were then analyzed using the Agilent TM RNA 6000 Pico Kit (PN# 5067-1513) on a 2100 Bioanalyzer TM following manufacturers protocol.

    Incubation:

    Article Title: Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT
    Article Snippet: .. An in vitro transcribed 7.6 kb HCV small genome RNA fragment was incubated in 1X reaction buffer for UltraMarathonRT ® (RNAConnect TM Cat. No. R1002), Maxima TM H Minus (ThermoScientific TM , Cat#K1652), SuperScript TM IV (Invitrogen TM , Cat#18091300), and Induro ® RT (NEB TM , CatM0681) at their recommended reaction temperatures for 0, 10, 20, and 30 min respectively. .. Samples were then analyzed using the Agilent TM RNA 6000 Pico Kit (PN# 5067-1513) on a 2100 Bioanalyzer TM following manufacturers protocol.

    Reverse Transcription:

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification
    Article Snippet: To confirm the promoter strengths, RNA was extracted from 1mL of log phase cells from each strain with Monarch Total RNA Extraction Kit (NEB, T2010). .. 1 μg of the extracted RNA was reverse transcribed using Induro RT (NEB, M0681) and random hexamers. .. RT reactions were then diluted 1:10 and 5 μL of the dilution was used in 20 μL qPCR reaction with 250 nM oNEB-573 and 250 nM oNEB-574 primers and 2X Luna Universal qPCR Master Mix (NEB, M3003). tufA expression level was used as an internal control.

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification
    Article Snippet: Total RNA was extracted from log-phase E. coli with Monarch Total RNA Extraction Kit (NEB, T2010). .. The on-column DNase I treatment step was skipped to preserve the cDNA- TR -RNA hybrid. cDNA sequencing : To sequence the cDNA within cDNA- TR hybrids, 1 μg of the extracted RNA was reverse transcribed using Induro RT (NEB, M0681) with primers that bind to the 3’ end of the cDNA. ..

    Article Title: Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT
    Article Snippet: The complete library preparation protocol currently recommended by Oxford Nanopore Technologies (ONT) can be found here: https://nanoporetech.com/document/direct-rna-sequencing-sqk-rna004#overview-of-the-protocol In short, the ONT reverse transcription adapter is first ligated to poly(A) tailed RNA using NEB TM T4 DNA Ligase (NEB TM , cat#M0202M). .. Reverse transcription is then performed using Induro ® RT (NEB TM , CatM0681) at 60°C for 30 minutes followed by 70°C for 10 minutes to inactivate enzyme. .. The cDNA/RNA duplex is then purified using RNAClean XP beads (Beckman Coulter ® , Cat#A63987).

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.
    Article Snippet: Gels were imaged using a Cytivia Amersham Typhoon in fluorescence mode with the Alexa488 filter. .. 1 μg of the extracted RNA was reverse transcribed using Induro RT (NEB, M0681) and random hexamers. .. Cells were harvested by centrifugation, and genomic DNA was extracted from cell pellets using Monarch Spin gDNA Extraction kit (NEB, T3010) and used as the template to amplify VR4 for deep sequencing.

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer. ..

    other:

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.
    Article Snippet: PCR were carried out with Q5 DNA Polymerase (NEB, M0491) using an equimolar mixture of forward primers (oNEB-145, 170, 171, 172) and an equimolar mixture of reverse primers (oNEB-173, 174, 175, 176).

    Article Title: An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded Protein
    Article Snippet: MMLV RT (RNase H-) (Promega M5301) and Induro RT (NEB M0681S) were diluted to 10 U/μL in RT Buffer (50 mM Tris-HCl pH 7.5, 50 mM KCl, 5 mM MgCl 2 , and 0.05% Tween-20).

    Sequencing:

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification
    Article Snippet: Total RNA was extracted from log-phase E. coli with Monarch Total RNA Extraction Kit (NEB, T2010). .. The on-column DNase I treatment step was skipped to preserve the cDNA- TR -RNA hybrid. cDNA sequencing : To sequence the cDNA within cDNA- TR hybrids, 1 μg of the extracted RNA was reverse transcribed using Induro RT (NEB, M0681) with primers that bind to the 3’ end of the cDNA. ..

    Purification:

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer. ..



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